Coding

Part:BBa_K215261:Design

Designed by: Alex Leone   Group: iGEM09_Washington   (2009-10-01)


Lpp + 5tmr OmpA + GS Linker + TEV site + WT Streptavidin + 6His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 535
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 577
    Illegal AgeI site found at 628
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

See BBa_K215201 for the method used to put WT streptavidin in the OmpA surface display construct.


Source

BBa_K215200 + BBa_J36841

References